In vitro propagation and microtuber induction in Dioscorea belophylla (Prain) Voigt ex Haine

Authors

  • Sreeja Thankappan Central Tuber Crops Research Institute (ICAR-CTCRI), Sreekariyam, Thiruvananthapuram - 695017, Kerala, India
  • Abraham K Central Tuber Crops Research Institute (ICAR-CTCRI), Sreekariyam, Thiruvananthapuram - 695017, Kerala, India

Keywords:

Dioscorea belophylla, in vitro propagation, microtuber induction, BAP, Kin, NAA

Abstract

A protocol was developed for the in vitro propagation and microtuber induction in Dioscorea belophylla  using nodal explants at Central Tuber Crops Research Institute, Thiruvananthapuram, India. MS  medium with benzyl adenine (BA) at 3 μM and Kinetin (Kin) at 4 and 5 μM were found to be the best  concentrations for developing shoots with good number of nodes and leaves. The addition of 1-naphthaleneacetic acid (NAA) along with cytokinin resulted in healthier leaf production. Among the  different concentrations of sucrose tested, MS medium with 4% sucrose was the best for shooting,  having the highest shoot length of 11.8 cm and mean number of 17.4 nodes and 11.4 leaves/shootwithin 15.4 days of inoculation. Microtuber induction was recorded in the presence of BA (3-5 μM),  Kin (3-4 μM) and BA 3 μM along with NAA (1 μM). Addition of 3-8% sucrose in the medium was found  to be the best concentration for inducing 1-3 microtubers/culture.

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Published

2015-01-16

How to Cite

Thankappan, S., & K, A. (2015). In vitro propagation and microtuber induction in Dioscorea belophylla (Prain) Voigt ex Haine. JOURNAL OF ROOT CROPS, 40(1), 31–38. Retrieved from https://ojs338.isrc.in/index.php/jrc/article/view/230

Issue

Vol. 40 No. 1 (2014): Journal of Root Crops

Section

Research Articles

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