Identification of disease resistance genes in cassava using NBS transcriptome profiling
Keywords:
Key words, cassava mosaic disease, NBS transcritome profiling, Lipid-transfer protein, heat shock proteinsAbstract
AbstractCassava production is seriously affected by cassava mosaic disease (CMD) and is one of the most important problems in India and Africa. The disease can be kept under control by deploying resistance genes through marker assisted selection (MAS). Nucleotide Binding Site (NBS) profiling is a motif directing profiling technique specifically targets chromosomal region containing resistance genes (R) and produce markers tightly linked withR-genes. The present study aims to target disease resistance (R) genes in cassava using NBS transcriptome profiling technique. For this purpose, two cassava varieties viz., MNga-1 (resistant variety) and CI732 (susceptible variety) were selected on the basis of CMD resistance and generated reproducible polymorphic multi-locus banding pattern with NBS prmers (NBS2 &NBS5). The polymorphic bands present in the resistant variety were eluted and directly sequenced using ABI3500 automated sequencer. The functional annotations of the sequences were carried out in both Phytozome v10 and NCBI using blastn program and RCa6 gene, non-specific lipid-transfer protein (ns-LTPs) and heat shock proteins (Hsp 90, Hsp 89-1, Hsp 81.2) involved in plant defense against cassava mosaic virus and its insect vector whitefly.References
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