Rapid Method for Determining Total Chlorophyll, Chlorophyll a and b and Carotene Content in Leaves of Elephant Foot Yam Using SPAD Meter

Authors

  • V Ravi Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram 695 017, Kerala, India
  • C Hridya Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram 695 017, Kerala, India
  • S Suchitra Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram 695 017, Kerala, India
  • G Byju Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram 695 017, Kerala, India

Abstract

A rapid, non-destructive method for determining total chlorophyll, chlorophyll a and b and carotene content in leaves of elephant foot yam using SPAD meter readings was developed at Central Tuber Crops Research Institute, Thiruvananthapuram, India. There was significant positive correlation between SPAD readings and total chlorophyll (r = 0.91), chlorophyll a (r = 0.85) and b (r = 0.90) and carotene (r = 0.86) content in leaves of elephant foot yam. The factors (F values) derived between SPAD readings and total chlorophyll, chlorophyll a and b and carotene content were 0.0275, 0.019,0.0087 and 0.0105 respectively. The coefficients of determination (r2) values derived between SPAD readings and total chlorophyll, chlorophyll a and b and carotene content were 0.833, 0.713, 0.813 and 0.736 respectively. The linear regression equations (y = a + bx) derived between SPAD readings and total chlorophyll, chlorophyll a and b and carotene content were 0.028x . 0.047, 0.02x . 0.44,0.008x + 0.011 and 0.006x + 0.125 respectively. The coefficient of determination (r2) values between SPAD readings and total chlorophyll, chlorophyll a and b and carotene content near to 1 indicated that the regression equations were good fit. Therefore, using the F values, the total chlorophyll, chlorophyll a and b and carotene content in leaves of elephant foot yam can be directly calculated by multiplying the SPAD readings with F values 0.0275, 0.019, 0.0087 and 0.0105 respectively.

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Published

2013-07-23

How to Cite

Ravi, V., Hridya, C., Suchitra, S., & Byju, G. (2013). Rapid Method for Determining Total Chlorophyll, Chlorophyll a and b and Carotene Content in Leaves of Elephant Foot Yam Using SPAD Meter. JOURNAL OF ROOT CROPS, 37(1), 29–31. Retrieved from https://ojs338.isrc.in/index.php/jrc/article/view/5

Issue

Vol. 37 No. 1 (2011): Jounal of Root Crops 37(1)

Section

Research Articles

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