Influence of Age of Explants and Genotype on Somatic Embryogenesis in African and Indian Cassava Cultivars

Authors

  • Beena M Ravindran SRF, CTCRI Present Address Post Doctoral Fellow, DSMZ, Braunschweig-38104
  • Stephan Winter Head, Plant Virology Department, Leibniz Institute-DSMZ, Braunschweig-38104
  • Makeshkumar Thangaraj Principal Scientist, Central Tuber Crops Research Institute, Sreekariym, Trivandrum - 695017

Keywords:

Cassava, African & Indian cultivar, embryogenic callus, somatic embryos

Abstract

Potential of eight cassava cultivars viz., TME3, TME4, TMS 30572, TMS 1089A, 96/0160, Albert,Kibaha and H165 for initiating somatic embryos was studied. Two different explants, young leaf lobe pre-cultured on Murashige and Skoog (MS) medium and axillary bud pre-cultured on MS medium containing BAP were tried. The age of explants and type of cultivar highly influenced the frequency and quantity of somatic embryos. Axillary buds of cv. Albert and young leaf lobes of cv. H165 responded well, while the same results were observed for conversion and development. Hardening and establishment in green house was close to 100% for all the cultivars under study. This paper discusses the effect of juvenility of the explants used, duration of pre-treatments and culture on embryogenesis medium, cultivar and its preference of explant, health of the mother plants, on successful regeneration of cassava via somatic embryogenesis, which could be very resourceful for friable embryogenic calli production and successful genetic transformation.

References

Atehnkeng. J., Adetimirin V. O., Ng S. Y. C. 2006. Exploring the African cassava (Manihot esculenta Crantz) germplasm for somatic embryogenic competence. Afr. J. Biotechnol., 5(14):1324.1329.

Bocks K. K., Wood R. D. 1983. The etiology of African cassava mosaic virus disease. Plant Disease 67:994-995.

Bull S. E., Owiti J. A., Niklaus M., Beeching J. R., Gruissem W., Vanderschuren H. 2009. Agrobacterium-mediated transformation of friable embryogenic calli and regeneration of transgenic cassava. Nat. Protoc., 4:1845- 54.

Chitundu M., Droppelmann K., Haggblade S. 2006. A value chain task force approach for managaing private . public partnerships: Zambia’s task force on acceleration of cassava utilization. Working paper No. 20, Food Security Research Project, Lusaka, Zambia.

Edison S., Anantharaman M., Srinivas T. 2006. Status of cassava in India an overall view. CTCRI Technical Bulletin Series: 46 pp 11-17.

Feitosa T., Bastos J. L. P., Ponte L. F. A., Jucá T. L., Campos F. A. P. 2007. Somatic embryogenesis in cassava genotypes from the northeast of Brazil. Braz. Arch. Biol. Technol., 50:201.206.

Gondwe T., Mvula W. M., Kalimbira A. A. 2001. Potential of utilizing cassava in animal feeds. In: Cassava commercialization for economic development in Malawi. Mahungu N. M., Banda J. W., Mattya C. (Ed.). Proceedings of the Symposium held at kwacha International Conference Center, Blantyre Malawi, May 2001

Groll J., Mycock D. J., Gray V. M., Laminski S. 2001. Secondary somatic embryogenesis of cassava on picloram supplemented media. Plant Cell, Tiss. Org. Cult., 65:201.210.

Guohua M. 1998. Effects of cytokinins and auxins on cassava shoot organogenesis and somatic embryogenesis from somatic embryo explants. Plant Cell, Tiss. Org. Cult. 54:1.7.

Guohua M., Qiusheng X. 2002. Induction of somatic embryogenesis and adventitious shoots from immature leaves of cassava. Plant Cell, Tiss. Org. Cult. 70:281.288.

Guthrie E. J. 1987. African cassava mosaic disease and its control. In International Seminar on African Cassava Mosaic Disease and its Control, Yamoussoukro. Ivory Coast, 4 - 8 May. pp. I - 9. Wageningen: eTA.

Haggblade S., Nyembe M. 2007. Preliminary draft Zambian Cassava Markets. Paper prepared for the acceleration of cassava utilization (ACU) task force meeting, February 1, 2007. Food Security Research Project

Hankoua B. B., Ng S. Y. C., Fawole I., Puonti-Kaerlas J., Pillay M., Dixon A. G. O. 2005. Regeneration of a wide range of African cassava genotypes via shoot organogenesis from cotyledons of maturing somatic embryos and conformity of the field-established regenerants. Plant Cell, Tiss. Org. Cult., 82:221.231.

Hankoua B. B., Taylor N. J., Ng S. Y. C., Fawole I., Puonti-Kaerlas J., Padmanabhan C., Yadav J. S., Fauquet C. M., Dixon A. G. O., Fondong V. N. 2006. Production of the first transgenic cassava in Africa via direct shoot organogenesis from friable embryogenic calli and germination of maturing somatic embryos. African J. Biotechnol. 5:1700.1712.

Hoagland D. R., Arnon D. I. 1938. The water culture method for growing plants without soil. Circular 347, College of Agriculture, Univ. of Calif.

Konan N. K., Sangwan R. S., Sangwan-Norreel B. S. 1994. Somatic embryogenesis from cultured mature cotyledons of cassava (Manihot esculenta Crantz) - identification of parameters influencingthe frequency of embryogenesis. Plant Cell, Tiss. Org. Cult. 37:91-102.

Li H. Q., Guo J. Y., Huang Y. W., Liang C. Y., Liu H. X., Potrykus I., Puonti-Kaerlas J. 1998. Regeneration of cassava plants via shoot organogenesis. Plant Cell Rep. 17:410.414.

Liu J., Zheng Q., Ma Q., Kranthi Kumar G., Zhang P. 2011. Cassava Genetic Transformation and its Application in Breeding. J. Integ. Plant Biol. 53(7):552-569.

Munyikwa T.R.I., Raemakers C. J. J. M., Schreuder M., Kok R., Rozeboom M., Jacobsen E. and Visser R. G. F. 1998. Pinpointing towards improved transformation and regeneration of cassava. Plant Sci. 135: 87.101.

Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15:473.497.

Mussio I., Chaput M. H., Serraf I., Ducreux G., Sihachakr D. 1998. Adventitious shoot regeneration from leaf explants of an African clone of cassava (Manihot esculenta Crantz) and analysis of the conformity of regenerated plants. Plant Cell, Tiss. Org. Cult. 53:205.211.

Ogburia M. N. 2003. Somatic embryogenesis, plantlet regeneration and micropropagation of cultivars and F1 hybrids of Manihot esculenta. Biol. Plant. 47(3): 429-432.

Putten H. J. J. K., Sudarmonowati E., Herman M., Pereira-Bertram I. J., Wolters A. M. A., Meima H., de Vetten N., Raemakers C. J. J. M., Visser R. G. F. 2012. Field testing and exploitation of genetically modified cassava with low-amylose or amylose-free starch in Indonesia. Trans. Res. 21:39.50.

Raemakers C. J. J. M., Bessembinder J., Staritsky G., Jacobsen E., Visser R. G. F. 1993. Induction, germination and shoot development of somatic embryos of cassava. Plant Cell, Tiss. Org. Cult. 33:151.156.

Raemakers C. J. J. M., Sofiari E., Jacobsen E., Visser R. G. F. 1997. Regeneration and transformation in cassava. Euphytica 96: 153.161.

Raemakers K., Evert Jacobsen, Richard Visser 2000. The Use of Somatic Embryogenesis for Plant Propagation in Cassava. Mol. Biotechnol. 14:215-221.

Raemakers K., Schreuder M., Pereira I., Munyikwa T., Jacobsen E., Visser R. 2001. Progress made in FEC transformation of cassava. Euphytica 120:15-24.

Saelim L., Phansiri S., Netrphan S., Suksangpanomrung M., Narangajavana J. 2006. Optimization of in vitro cyclic somatic embryogenesis and regeneration of the Asian cultivars of cassava (Manihot esculenta crantz) for genetic manipulation system. Global J. Biotechnol. Biochem. 1:7.15

Schopke A. E. G., Schopke C., Taylor N. J., Beachy R. N., Fauquet C. M. 1998. Regeneration of transgenic plants (Manihot esculenta Crantz) through Agrobacterium-mediated transformation of embryogenic suspension cultures. Plant Cell Rep. 17:827.831.

Sofiari E., Raemakers C. J. J. M., Kanju E., Danso K., Van Lammeren A. M., Jacobsen E., Visser R. G. F., 1997. Comparison of NAA and 2, 4-D induced somatic embryogenesis in cassava. Plant Cell, Tiss. Org. Cult. 50:45.56.

Stamp J. A., Henshaw G. G. 1982. Somatic embryogenesis in cassava. Zeitschrift Pflanzenphysiol. 105:183.187

Stamp J. A., Henshaw G. G. 1987. Somatic embryogenesis from clonal leaf tissue of cassava. Ann. Bot. 59:445-450.

Szabados L., Hoyos R., Roca W. 1987. In vitro somatic embryogenesis and plant regeneration of cassava. Plant Cell Rep. 6:248.251.

Taylor N., Gaitán-Solís E., Moll T., Trauterman B., Jones T., Pranjal A., Trembley C., Abernathy V., Corbin D., Fauquet C. M. 2012. A High-throughput Platform for the Production and Analysis of Transgenic Cassava (Manihot esculenta) Plants. Tropical Plant Biol. 5:127.139.

Taylor N. J., Masona M. V., Carcamo R., Ho T., Schöpke C., Fauquet C. M. 2001. Production of embryogenic tissue and regeneration of transgenic plants in cassava (Manihot esculenta Crantz). Euphytica 120:25.34.

Thro A. M., Fregene M., Taylor N. J., Raemakers C. J. J. M., Pounti-Kaerlas J., Schopke C., Visser R. G. F., Potrykus I., Fauquet C. M., Roca W., Hershey C. 1999. Genetic biotechnologies and cassava-based development. In: Gene- and Biotechnology of Food Crops in Developing Countries. Hohn T. and Leisinger K. (Ed.), Springer-Verlag, Berlin. pp. 142-175.

Vanderschuren H., Akbergenov R., Pooggin M. M., Hohn T., Gruissem W., Zhang P. 2007. Transgenic cassava resistance to African cassava mosaic virus is enhanced by viral DNA-A bidirectional promoter-derived siRNAs. Plant Mol. Biol. 64:549-57.

Zhang P., Legris G., Coilin P., Puonti-Kaerlas J. 2000. Production of stably transformed cassava plants via particle bombardment. Plant Cell Rep. 19:939.945.

Zhang P., Phansiri S., Puonti-Kaerlas J. 2001. Improvement of cassava shoot organogenesis by the use of silver nitrate in vitro. Plant Cell, Tiss. Org. Cult. 67:47.54.

Zhang P., Puonti-Kaerlas J. 2005. Regeneration of transgenic cassava from transformed embryogenic tissues. In: Transgenic Plants: Methods and Protocols. Peña, L. (Ed.), Humana press Inc., New Jersey, pp. 165.176.

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Published

2015-01-19

How to Cite

Ravindran, B. M., Winter, S., & Thangaraj, M. (2015). Influence of Age of Explants and Genotype on Somatic Embryogenesis in African and Indian Cassava Cultivars. JOURNAL OF ROOT CROPS, 40(2), 21–27. Retrieved from https://ojs338.isrc.in/index.php/jrc/article/view/161

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Research Articles