Comparative potential of Somatic embryogenesis and Friable Embryogenic Callus production in farmer preferred Indian cassava cultivars
Keywords:
cassava, friable embryogenic callus, Indian cultivar, somatic embryogenesisAbstract
The most crucial step towards developing farmer preferred qualities in cassava is to successfully produce stable and re-generable friable embryogenic callus (FEC). FEC production in four Indian cassava cultivars viz., H226, H165, Sree Vijaya, Sree Sahya in comparison with model cultivar, TMS 60444 were attempted. The focus was also on production and maintenance of virus free in vitro mother plants, optimization of somatic embryogenesis using two different explants, axillary bud (AB) and immature leaf lobe (ILL), other than induction of FEC and evaluation of its regeneration potential. All the varieties produced a moderate to optimum OES production on 12mg/l Picloram, in which the highest frequency of OES induction of 97.49 % and 95.64 % were obtained in H226 and H165, respectively using ILL. The number of healthy plantlets regenerated from 50 mg of FEC was 14, 11, and 17 from H165, H226 and TMS 60444 respectively. These results could be a pointer towards the production of FEC in other Indian cassava varieties, which are highly susceptible to CMD with suitable modification in culture media for developing resistance.References
Abraham, K., Nair, S. G. and Naskar, S. K. 2000. Cassava breeding and varietal dissemination in India - Major achievements during the past 25-30 years. In Sixth Asian Cassava Research Workshop. 21-25 February 2000. Vietnam. pp 176-181.
Adejare, G. O. and Coutts, R. H. 1981. Eradication of cassava mosaic disease from Nigerian cassava clones by meristem-tip cultivation. Plant Cell, Tiss. Org. Cult., 1: 25-32.
Anitha J., Makeshkumar, T. and Edison, S. 2011. Survey of cassava mosaic disease in Kerala. J. Root crops, 37(1): 41-47.
Anuradha, T., Kumar, K. K. and Balasubramanian, P. 2015. Cyclic somatic embryogenesis of elite Indian cassava variety, H226. Indian J. Biotechno., 14: 559-565.
Atehnkeng, J., Adetimirin, V.O. and Ng, S.Y.C., 2006. Exploring the African cassava (Manihot esculenta Crantz) germplasm for somatic embryogenic competence. Afri. J. Biotechno. 5, 1324-1329.
Beena, M. R., Winter, S. and Makeshkumar, T. 2014. Influence of age of explants and genotypes on somatic embryogenesis in African and Indian cassava cultivars. J. Root crops, 40(2): 21-27.
Berbee, F.M., Berbee, J.G. and Hildebrandt, A.C. 1973. Induction of callus and virus symptomless plants from shoot tip cultures of cassava. Invitro, 8:421.
Bull, S.E., Owiti, J.A., Niklaus, M., Beeching, J.R., Gruissem, W., and Vanderschuren, H. 2009. Agrobacterium-mediated transformation of friable embryogenic calli and regeneration of transgenic cassava. Nat. Protoc., 4: 1845.1854.
Chavarriaga-Aguirre, P., Brand, A., Medina, A., PrÃas, M., Escobar, R., Martinez, J., DÃaz, P., López, C., Roca, W. M. and Tohme, J. 2016. The potential of using biotechnology to improve cassava: a review. In Vitro Cell. Dev. Biol. . Plant. DOI 10.1007/s11627-016-9776-3.
Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem., 19: 11-15.
Food and Agricultural Organisation. 2015. Cassava area harvested, yield and production data, 2014.
Gresshoff, P. and Doy, C. 1974. Derivation of a haploid cell line from Vitis vinifera and the importance of the stage of meiotic development of anthers for haploid culture of this and other genera. Z. Pflanzen physiol., 73,132.141.
Hankoua, B.B., Ng, S.Y.C., Fawole, I., Puonti-Kaerlas, J., Pillay, M. and Dixon, A.G.O. 2005. Regeneration of a wide range of African cassava genotypes via shoot organogenesis from cotyledons of maturing somatic embryos and conformity of the field-established regenerants. Plant Cell Tiss. Org. Cult., 82: 221-231.
Hankoua, B. B., Taylor, N. J., Ng, S.Y.C., Fawole, I., Puonti-Kaerlas, J., Padmanabhan, C., Yadav, J.S., Fauquet, C.M., Dixon, A.G.O. and Fondong, V.N., 2006. Production of the first transgenic cassava in Africa via direct shoot organogenesis from friable embryogenic calli and germination of maturing somatic embryos. Afr. J. Biotechnol., 5: 1700.1712.
ICAR-CTCRI. Annual Report 2015 - 2016, ICAR-Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala, India, pp.168.
Jayakrishnan, J.T. and Makeshkumar, T. 2016. Molecular phylogeography of cassava mosaic viruses in Kerala. In: National Conference on Tropical Tuber Crops for the Sustenance and welfare of tribal communities- 2016, Conference document. Sheela Immanuel (Eds). St. Josephs Press, Thiruvananthapuram, pp. 234.
Jos, J.S., N. Hrishi, S. B. Maini and R. G. Nair. 1981. Two high yielding hybrids of cassava. J. Root Crops, 6: 1-6.
Joseph, R., Yeoh, H. H. and Loh, C. S. 2004. Induced mutations in cassava using somatic embryos and the identification of mutant plants with altered starch yield and composition. Plant Cell Rep., 23(1/2): 91-98.
Kaiser, W. J. and Teemba, L. R. 1979. Use of tissue culture and thermotherapy to free East African cassava cultivars of African cassava mosaic and cassava brown streak diseases. Plant Disease Reporter, 63: 780-784.
Kartha, K.K. and Gamborg, O. L. 1974. Elimination of cassava mosaic disease by meristem culture. Phytopath., 65: 826-828.
Kartha, K.K., Gamborg, O. L., Constabel, F. and Shyluk, J. P. 1975. Regeneration of plants through shoot apical meristems. Plant Sci. Lett., 2: 107-113.
Liu, J., Zheng, Q., Ma, Q., Gadidasu K.K., Zhang, P. (2011) Cassava Genetic Transformation and its Application in Breeding. J. Integr. Plant Biol., 53: 552.569.
Magoon, M. L., S. G. Appan, R. Krishnan and R. C. Mandal. 1970. Some promising high yielding hybrids and selections of cassava. SABRAO Newsletter 2: 19-26.
Malathi, V. G., Nair, N. G. and Santha, P. 1985. Cassava mosaic disease. Central Tuber Crops Research Institute Technical bulletin series, 5: 18.
Makeshkumar, T., Anoopsankar, Nair, R. R. and Edison, S. 2005. Detection of Indian cassava mosaic virus through Polymerase chain reaction and Nucleic acid Hybridisaton techniques. J. Root crops, 31(1): 1-6.
Makeshkumar, T., Dhanya J., Beena, M. R., Prakash Krishnan, B.S. and Winter, S. 2012. Studies to develop RNAi mediated resistance against cassava mosaic viruses in India. In: Second Scientific Conference of Global Cassava Partnership for the 21st Century, Oral and Poster presentation - GCP21, Kampala, Uganda.
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant., 15(3): 473-497
Nair, N. G. 1990. Performance of virus - free cassava (Manihot esculenta Crantz.) developed through meristem tip culture. J. Root Crops, 16(2): 123 . 131.
Nair, N. G. and Chandrababu, S. 1996. In vitro production and micropropagation of three species of edible yams. In: Tropical Tuber Crops: Problems, Prospects and Future Strategies. Kurup, G. T., Palaniswami, M.S., Potty, V.P., Padmaja, G., Kabeerathumma, S. and Pillai, S. V. (Eds.). Oxford and IBH Publishing, New Delhi. pp. 211-215.
Nkaa, F. A., Ene-Obong, E. E., Taylor, N. J., Fauquet, C. M. and Mbanaso, E.N.A. 2013. Elimination of African Cassava Mosaic Virus (ACMV) and East African Cassava Mosaic Virus (EACMV) from Cassava (Manihot esculenta Crantz) cv. ‘Nwugo’ via somatic embryogenesis. Am. J. Biotechnol. Mol. Sci., 3(2): 33-40.
Nkaa, F. A., Ene-Obong, E. E., Taylor, N. J., Fauquet, C. M. and C. N. Egesi. 2015. Evaluation of ten elite Nigerian cassava (Manihot esculenta Crantz) cultivar for somatic embryogenesis and regeneration potentials. J. Adv. Biol. Biotechnol., 2(2): 133-143.
Nyaboga, E., Njiru,J., Nguu, E., Gruissem, W., Vanderschuren, H., and Tripathi, L. 2013. Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer- preferred cassava cultivars. Front. Plant Sci., 4: 526.
Nyaboga, E., Njiru, J., and Tripathi, L., 2015. Factors influencing somatic embryogenesis, regeneration, and Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) cultivar TME14. Front. Plant Sci., 6: 411.
Otono, F. B., Ndofunsu, A. D., Nakweti, R. K., Ntumbula, A. M., Hity, M., Kasali, L., Ndiku, L., Gladys, R. and Philippe, L. . 2015. PCR and ELISA detection of cassava mosaic virus in a Congolese cassava landrace. Internat. J. Biotechnol. Food Sci., 3(1):10-16.
Patil, B. L., Rajasubramaniam, S., Bagchi, C. and Dasgupta I. 2005. Both Indian cassava mosaic virus and Sri Lankan cassava mosaic virus are found in India and exhibit high variability as assessed by PCR-RFLP. Arch Virol., 150: 389-397.
Pinheiro, M. V. M. 2013. Maturation of Anthurium andraeanum cv. Eidibel somatic embryos from nodal segments. In Vitro Cell. Dev. Bio. Plant. 49(3): 304.312.
Raemakers, K., Schreuder, M., Pereira, I., Munyikwa, T., Jacobsen, E. and Visser, R. 2001. Progress made in FEC transformation of cassava. Euphytica, 120: 15-24.
Rajanimala, N., Rabindran, R., Mohan, S., and Sethuraman, K. 2011. Survey for the occurrence of Cassava mosaic Disease in Tamil Nadu. J Root Crops, 37(2): 197-199.
Rossin, C.B. and Rey, M.E.C. 2011. Effect of explant source and auxins on somatic embryogenesis of selected cassava (Manihot esculenta Crantz) cultivars. South Afri. J. Bot., 77: 59.65.
Saelim, L., Phansiri, S., Netrphan, S., Suksangpanomrung, M. and Narangajavana, J. 2006. Optimization of in vitro cyclic somatic embryogenesis and regeneration of the Asian cultivars of cassava (Manihot esculenta Crantz) for genetic manipulation system. Global J. Biotechnol. Biochem., 1: 7-15.
SAS. 2010. SAS/STAT Software Version 9.3, SAS Institute Inc. Cary, NC.
Sheela, M. N., Abhilash, P. V., Dharshan, S., Prakash Krishnan, B.S, Arun, P., Makeshkumar, T., Asha, K.I., Mohan, C., Sunitha, S., Krishna Radhika, N., Asha Devi, A. and James George. 2016. Breeding for varieties resistant to cassava mosaic disease using different source of resistance. In: National Conference on Tropical Tuber Crops for the Sustenance and welfare of tribal communities- 2016 Conference document. Sheela Immanuel (Eds.). St. Josephs Press, Thiruvananthapuram. pp. 42.
Szabados, L., Hoyos, R. and Roca, W. 1987. In vitro somatic embryogenesis and plant regeneration of cassava. Plant Cell Rep., 6(3): 248-251.
Taylor, N.J., Edwards, M., Kiernan, R.J., Davey, C.D., Blakesley, D. and Henshaw, G.G. 1996. Development of friable embryogenic callus and embryogenic suspension culture systems in cassava (Manihot esculenta Crantz). Nat Biotechnol., 14(6): 726-730.
Taylor, T., Gaitán-SolÃs, E., Moll, T., Trauterman, B., Jones, T., Pranjal, A., Trembley, C., Abernathy, V., Corbin, D. and Fauquet, C. M. 2012. A high-throughput platform for the production and analysis of transgenic cassava (Manihot esculenta) plants. Trop. Plant Biol., 5: 127-139.
Thankappan, M., Makeshkumar, T. and Edison, S. 1997. Management of virus diseases in tropical tuber crops. Proceedings of the International Conference on Integrated PlantDisease Management for Sustainable Agriculture, Indian Phytopathological Society, pp. 1173-1175.
Ubalua, A. O. and Mbanaso, E. 2014. Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444). J. Evol.Biol. Res., 6(3): 9-12.
Vidal, Ã. M., de Carvalho C. M. A. P., da Silva, S.A., de Almeida, W. A. B., and Souza, F. V. D. 2014. In vitro regeneration and morphogenesis of somatic embryos of cassava. Revista Ciência Agronômica, 45(3): 558-565.
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