Micropropagation for Rapid Multiplication of Planting Material in Cassava (Manihot esculenta Crantz)

Authors

  • Shiji R Central Tuber Crops Research Institute (ICAR-CTCRI), Sreekariyam, Thiruvananthapuram - 695017, Kerala, India
  • James George Project Coordinator - AICRP on Tuber Crops Central Tuber Crops Research Institute (ICAR-CTCRI), Sreekariyam, Thiruvananthapuram - 695017, Kerala, India
  • Sunitha S Principal Scientist - AICRP on Tuber Crops Central Tuber Crops Research Institute (ICAR-CTCRI), Sreekariyam, Thiruvananthapuram - 695017, Kerala, India
  • Muthuraj R Senior Scientist - Seed Technology Central Tuber Crops Research Institute (ICAR-CTCRI), Sreekariyam, Thiruvananthapuram - 695017, Kerala, India

Keywords:

In vitro regeneration, micropropagation, BAP, NAA, hardening

Abstract

Rapid multiplication of planting material in cassava through tissue culture techniques such as shoot  initiation, micropropagation and hardening was studied in cassava variety Sree Padmanabha. The  effect of nodal segments from axillary bud of the field grown cassava cultured in Murashige and  Skoog’s basal medium supplemented with different concentrations and combinations of 6-Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) were evaluated based on different  parameters viz., number of shoots, number of roots, number of leaves and number of nodes. Minimal  risk of contamination was observed with sterilization in 0.1% HgCl2 for 7 min, which was found to be  an effective surface sterilant. For in vitro regeneration using apical node as explant, MS media with  0.3 mg l-1 NAA was found to be the best in terms of shoot having three nodes. When in vitro shoots  were inoculated in MS media with 0.1 mg l-1 NAA, rooting was the most profuse. Nodal explants took  one month for development into plantlets having three nodes. Potential number of planting material  production from one nodal explant using micropropagation techniques was estimated to be ranging  from 16,000 to 17,000 in one year period. Well rooted in vitro plantlets in sterilized vermiculite with  4-5 cm length were found suitable for hardening and subsequent transplanting, which gave 91%success after two months of hardening.

Author Biography

Muthuraj R, Senior Scientist - Seed Technology Central Tuber Crops Research Institute (ICAR-CTCRI), Sreekariyam, Thiruvananthapuram - 695017, Kerala, India

Se

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Published

2015-01-15

How to Cite

R, S., George, J., S, S., & R, M. (2015). Micropropagation for Rapid Multiplication of Planting Material in Cassava (Manihot esculenta Crantz). JOURNAL OF ROOT CROPS, 40(1), 23–30. Retrieved from https://ojs338.isrc.in/index.php/jrc/article/view/229

Issue

Vol. 40 No. 1 (2014): Journal of Root Crops

Section

Research Articles

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